c8 d1a murine astrocytic cell lines Search Results


c8 d1a murine cerebellar astrocytes  (ATCC)
96
ATCC c8 d1a murine cerebellar astrocytes
C8 D1a Murine Cerebellar Astrocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/pm37290222-60-42-46?v=ATCC
Average 96 stars, based on 1 article reviews
c8 d1a murine cerebellar astrocytes - by Bioz Stars, 2026-07
96/100 stars
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c8 d1a murine astrocytic cell lines  (Procell Inc)
86
Procell Inc c8 d1a murine astrocytic cell lines
C8 D1a Murine Astrocytic Cell Lines, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/pmc12915190-411-12-21?v=Procell+Inc
Average 86 stars, based on 1 article reviews
c8 d1a murine astrocytic cell lines - by Bioz Stars, 2026-07
86/100 stars
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pk  (ATCC)
96
ATCC pk
Pk, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/custom%40ccl-33%4042249111?v=ATCC
Average 96 stars, based on 1 article reviews
pk - by Bioz Stars, 2026-07
96/100 stars
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vero  (ATCC)
99
ATCC vero
Vero, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/custom%40ccl-81%4030886672?v=ATCC
Average 99 stars, based on 1 article reviews
vero - by Bioz Stars, 2026-07
99/100 stars
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murine n2a neuroblastoma  (ATCC)
99
ATCC murine n2a neuroblastoma
DCEF effects on neuronal elements. <t>N2a</t> mouse cells were differentiated into neuronal-like cells and various aspects relating to viability (A), morphology (B), and response (C) were assessed following exposure to increasing DCEFs (0, 25, 50, and 100V/m). (A) The total percentage of viable N2a cells (as measured in 3–4 replicates, n total=877–1900 cells) as well as the percentage of viable N2a cells specifically depicting neurites (5–6 replicates, n total=901–1134) was similar across all DCEFs applied (from 0 to 100V/m). (C) Protein concentration for GAP-43, a molecule expressed during axonal/neurite growth, was increased following a 24-hour stimulation of 25 and 50V/m (graph depicting average data of 4 replicates). (D) Measurements of total neurite length revealed significant elongation (5–6 replicates, n total=449–624) (E) as well as a shift of orientation towards the cathode (5–6 replicates, n total=449–624) when cells were stimulated at 25, 50, and 100V/m. The polar distribution of neurite angles (each dot represents the angle of a single neurite) demonstrates a significantly more prominent orientation towards the cathode at 50 and 100V/m. (F) Additionally, the 25 and 50 V/m DCEFs had a significant effect on the total length of the cell body (5–6 replicates, n total=493–688) when compared with cells exposed to 0 or 100V/m DCEF. (G) Polar distribution of cell body angles (each dot represents the angle of a single cell body) reveals that cell angles reorient parallel to the DCEF. (H-K) Photomicrographs of double phalloidin (F-actin, red) and DAPI (nucleus, blue) immunofluorescent staining illustrating the morphological and orientational changes in N2a cells in response to DCEFs (scale bar: 20 µm). (A-D, F) The values represent LS-means ± SEM. Statistical analyses were performed using ANOVA (split-plot-type) (A-D, F). (E) Kuiper’s Test of Uniformity and (G) chi-square test using grouped data (number of classes = 8 with identical divisions for interval 0–180°). *** P <.001 vs 0V/m; ## P <.01, ### P <.001 vs 25V/m; & P <.05, &&& P <.001 vs 50V/m. DCEF, direct current electrical field; GAP-43, growth associated protein-43; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LS, least square; OD, optical density; V/m, Volt/meter.
Murine N2a Neuroblastoma, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/pmc04376545-109-0-3?v=ATCC
Average 99 stars, based on 1 article reviews
murine n2a neuroblastoma - by Bioz Stars, 2026-07
99/100 stars
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bhk-21  (ATCC)
99
ATCC bhk-21
DCEF effects on neuronal elements. <t>N2a</t> mouse cells were differentiated into neuronal-like cells and various aspects relating to viability (A), morphology (B), and response (C) were assessed following exposure to increasing DCEFs (0, 25, 50, and 100V/m). (A) The total percentage of viable N2a cells (as measured in 3–4 replicates, n total=877–1900 cells) as well as the percentage of viable N2a cells specifically depicting neurites (5–6 replicates, n total=901–1134) was similar across all DCEFs applied (from 0 to 100V/m). (C) Protein concentration for GAP-43, a molecule expressed during axonal/neurite growth, was increased following a 24-hour stimulation of 25 and 50V/m (graph depicting average data of 4 replicates). (D) Measurements of total neurite length revealed significant elongation (5–6 replicates, n total=449–624) (E) as well as a shift of orientation towards the cathode (5–6 replicates, n total=449–624) when cells were stimulated at 25, 50, and 100V/m. The polar distribution of neurite angles (each dot represents the angle of a single neurite) demonstrates a significantly more prominent orientation towards the cathode at 50 and 100V/m. (F) Additionally, the 25 and 50 V/m DCEFs had a significant effect on the total length of the cell body (5–6 replicates, n total=493–688) when compared with cells exposed to 0 or 100V/m DCEF. (G) Polar distribution of cell body angles (each dot represents the angle of a single cell body) reveals that cell angles reorient parallel to the DCEF. (H-K) Photomicrographs of double phalloidin (F-actin, red) and DAPI (nucleus, blue) immunofluorescent staining illustrating the morphological and orientational changes in N2a cells in response to DCEFs (scale bar: 20 µm). (A-D, F) The values represent LS-means ± SEM. Statistical analyses were performed using ANOVA (split-plot-type) (A-D, F). (E) Kuiper’s Test of Uniformity and (G) chi-square test using grouped data (number of classes = 8 with identical divisions for interval 0–180°). *** P <.001 vs 0V/m; ## P <.01, ### P <.001 vs 25V/m; & P <.05, &&& P <.001 vs 50V/m. DCEF, direct current electrical field; GAP-43, growth associated protein-43; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LS, least square; OD, optical density; V/m, Volt/meter.
Bhk 21, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/custom%40ccl-10%4010%2E1101%2F824813?v=ATCC
Average 99 stars, based on 1 article reviews
bhk-21 - by Bioz Stars, 2026-07
99/100 stars
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hela  (ATCC)
99
ATCC hela
DCEF effects on neuronal elements. <t>N2a</t> mouse cells were differentiated into neuronal-like cells and various aspects relating to viability (A), morphology (B), and response (C) were assessed following exposure to increasing DCEFs (0, 25, 50, and 100V/m). (A) The total percentage of viable N2a cells (as measured in 3–4 replicates, n total=877–1900 cells) as well as the percentage of viable N2a cells specifically depicting neurites (5–6 replicates, n total=901–1134) was similar across all DCEFs applied (from 0 to 100V/m). (C) Protein concentration for GAP-43, a molecule expressed during axonal/neurite growth, was increased following a 24-hour stimulation of 25 and 50V/m (graph depicting average data of 4 replicates). (D) Measurements of total neurite length revealed significant elongation (5–6 replicates, n total=449–624) (E) as well as a shift of orientation towards the cathode (5–6 replicates, n total=449–624) when cells were stimulated at 25, 50, and 100V/m. The polar distribution of neurite angles (each dot represents the angle of a single neurite) demonstrates a significantly more prominent orientation towards the cathode at 50 and 100V/m. (F) Additionally, the 25 and 50 V/m DCEFs had a significant effect on the total length of the cell body (5–6 replicates, n total=493–688) when compared with cells exposed to 0 or 100V/m DCEF. (G) Polar distribution of cell body angles (each dot represents the angle of a single cell body) reveals that cell angles reorient parallel to the DCEF. (H-K) Photomicrographs of double phalloidin (F-actin, red) and DAPI (nucleus, blue) immunofluorescent staining illustrating the morphological and orientational changes in N2a cells in response to DCEFs (scale bar: 20 µm). (A-D, F) The values represent LS-means ± SEM. Statistical analyses were performed using ANOVA (split-plot-type) (A-D, F). (E) Kuiper’s Test of Uniformity and (G) chi-square test using grouped data (number of classes = 8 with identical divisions for interval 0–180°). *** P <.001 vs 0V/m; ## P <.01, ### P <.001 vs 25V/m; & P <.05, &&& P <.001 vs 50V/m. DCEF, direct current electrical field; GAP-43, growth associated protein-43; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LS, least square; OD, optical density; V/m, Volt/meter.
Hela, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/custom%40ccl-2%4035372261?v=ATCC
Average 99 stars, based on 1 article reviews
hela - by Bioz Stars, 2026-07
99/100 stars
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ca ski  (ATCC)
97
ATCC ca ski
DCEF effects on neuronal elements. <t>N2a</t> mouse cells were differentiated into neuronal-like cells and various aspects relating to viability (A), morphology (B), and response (C) were assessed following exposure to increasing DCEFs (0, 25, 50, and 100V/m). (A) The total percentage of viable N2a cells (as measured in 3–4 replicates, n total=877–1900 cells) as well as the percentage of viable N2a cells specifically depicting neurites (5–6 replicates, n total=901–1134) was similar across all DCEFs applied (from 0 to 100V/m). (C) Protein concentration for GAP-43, a molecule expressed during axonal/neurite growth, was increased following a 24-hour stimulation of 25 and 50V/m (graph depicting average data of 4 replicates). (D) Measurements of total neurite length revealed significant elongation (5–6 replicates, n total=449–624) (E) as well as a shift of orientation towards the cathode (5–6 replicates, n total=449–624) when cells were stimulated at 25, 50, and 100V/m. The polar distribution of neurite angles (each dot represents the angle of a single neurite) demonstrates a significantly more prominent orientation towards the cathode at 50 and 100V/m. (F) Additionally, the 25 and 50 V/m DCEFs had a significant effect on the total length of the cell body (5–6 replicates, n total=493–688) when compared with cells exposed to 0 or 100V/m DCEF. (G) Polar distribution of cell body angles (each dot represents the angle of a single cell body) reveals that cell angles reorient parallel to the DCEF. (H-K) Photomicrographs of double phalloidin (F-actin, red) and DAPI (nucleus, blue) immunofluorescent staining illustrating the morphological and orientational changes in N2a cells in response to DCEFs (scale bar: 20 µm). (A-D, F) The values represent LS-means ± SEM. Statistical analyses were performed using ANOVA (split-plot-type) (A-D, F). (E) Kuiper’s Test of Uniformity and (G) chi-square test using grouped data (number of classes = 8 with identical divisions for interval 0–180°). *** P <.001 vs 0V/m; ## P <.01, ### P <.001 vs 25V/m; & P <.05, &&& P <.001 vs 50V/m. DCEF, direct current electrical field; GAP-43, growth associated protein-43; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LS, least square; OD, optical density; V/m, Volt/meter.
Ca Ski, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/custom%40crl-1550%4035372261?v=ATCC
Average 97 stars, based on 1 article reviews
ca ski - by Bioz Stars, 2026-07
97/100 stars
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inactivated foetal calf serum  (DUTSCHER DOMINIQUE)
90
DUTSCHER DOMINIQUE inactivated foetal calf serum
DCEF effects on neuronal elements. <t>N2a</t> mouse cells were differentiated into neuronal-like cells and various aspects relating to viability (A), morphology (B), and response (C) were assessed following exposure to increasing DCEFs (0, 25, 50, and 100V/m). (A) The total percentage of viable N2a cells (as measured in 3–4 replicates, n total=877–1900 cells) as well as the percentage of viable N2a cells specifically depicting neurites (5–6 replicates, n total=901–1134) was similar across all DCEFs applied (from 0 to 100V/m). (C) Protein concentration for GAP-43, a molecule expressed during axonal/neurite growth, was increased following a 24-hour stimulation of 25 and 50V/m (graph depicting average data of 4 replicates). (D) Measurements of total neurite length revealed significant elongation (5–6 replicates, n total=449–624) (E) as well as a shift of orientation towards the cathode (5–6 replicates, n total=449–624) when cells were stimulated at 25, 50, and 100V/m. The polar distribution of neurite angles (each dot represents the angle of a single neurite) demonstrates a significantly more prominent orientation towards the cathode at 50 and 100V/m. (F) Additionally, the 25 and 50 V/m DCEFs had a significant effect on the total length of the cell body (5–6 replicates, n total=493–688) when compared with cells exposed to 0 or 100V/m DCEF. (G) Polar distribution of cell body angles (each dot represents the angle of a single cell body) reveals that cell angles reorient parallel to the DCEF. (H-K) Photomicrographs of double phalloidin (F-actin, red) and DAPI (nucleus, blue) immunofluorescent staining illustrating the morphological and orientational changes in N2a cells in response to DCEFs (scale bar: 20 µm). (A-D, F) The values represent LS-means ± SEM. Statistical analyses were performed using ANOVA (split-plot-type) (A-D, F). (E) Kuiper’s Test of Uniformity and (G) chi-square test using grouped data (number of classes = 8 with identical divisions for interval 0–180°). *** P <.001 vs 0V/m; ## P <.01, ### P <.001 vs 25V/m; & P <.05, &&& P <.001 vs 50V/m. DCEF, direct current electrical field; GAP-43, growth associated protein-43; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LS, least square; OD, optical density; V/m, Volt/meter.
Inactivated Foetal Calf Serum, supplied by DUTSCHER DOMINIQUE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/pmc06433660-30-30-35?v=DUTSCHER+DOMINIQUE
Average 90 stars, based on 1 article reviews
inactivated foetal calf serum - by Bioz Stars, 2026-07
90/100 stars
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bend.3  (ATCC)
99
ATCC bend.3
DCEF effects on neuronal elements. <t>N2a</t> mouse cells were differentiated into neuronal-like cells and various aspects relating to viability (A), morphology (B), and response (C) were assessed following exposure to increasing DCEFs (0, 25, 50, and 100V/m). (A) The total percentage of viable N2a cells (as measured in 3–4 replicates, n total=877–1900 cells) as well as the percentage of viable N2a cells specifically depicting neurites (5–6 replicates, n total=901–1134) was similar across all DCEFs applied (from 0 to 100V/m). (C) Protein concentration for GAP-43, a molecule expressed during axonal/neurite growth, was increased following a 24-hour stimulation of 25 and 50V/m (graph depicting average data of 4 replicates). (D) Measurements of total neurite length revealed significant elongation (5–6 replicates, n total=449–624) (E) as well as a shift of orientation towards the cathode (5–6 replicates, n total=449–624) when cells were stimulated at 25, 50, and 100V/m. The polar distribution of neurite angles (each dot represents the angle of a single neurite) demonstrates a significantly more prominent orientation towards the cathode at 50 and 100V/m. (F) Additionally, the 25 and 50 V/m DCEFs had a significant effect on the total length of the cell body (5–6 replicates, n total=493–688) when compared with cells exposed to 0 or 100V/m DCEF. (G) Polar distribution of cell body angles (each dot represents the angle of a single cell body) reveals that cell angles reorient parallel to the DCEF. (H-K) Photomicrographs of double phalloidin (F-actin, red) and DAPI (nucleus, blue) immunofluorescent staining illustrating the morphological and orientational changes in N2a cells in response to DCEFs (scale bar: 20 µm). (A-D, F) The values represent LS-means ± SEM. Statistical analyses were performed using ANOVA (split-plot-type) (A-D, F). (E) Kuiper’s Test of Uniformity and (G) chi-square test using grouped data (number of classes = 8 with identical divisions for interval 0–180°). *** P <.001 vs 0V/m; ## P <.01, ### P <.001 vs 25V/m; & P <.05, &&& P <.001 vs 50V/m. DCEF, direct current electrical field; GAP-43, growth associated protein-43; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LS, least square; OD, optical density; V/m, Volt/meter.
Bend.3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/custom%40crl-2299%4010%2E1101%2F2021%2E11%2E24%2E469889?v=ATCC
Average 99 stars, based on 1 article reviews
bend.3 - by Bioz Stars, 2026-07
99/100 stars
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mdck  (ATCC)
99
ATCC mdck
DCEF effects on neuronal elements. <t>N2a</t> mouse cells were differentiated into neuronal-like cells and various aspects relating to viability (A), morphology (B), and response (C) were assessed following exposure to increasing DCEFs (0, 25, 50, and 100V/m). (A) The total percentage of viable N2a cells (as measured in 3–4 replicates, n total=877–1900 cells) as well as the percentage of viable N2a cells specifically depicting neurites (5–6 replicates, n total=901–1134) was similar across all DCEFs applied (from 0 to 100V/m). (C) Protein concentration for GAP-43, a molecule expressed during axonal/neurite growth, was increased following a 24-hour stimulation of 25 and 50V/m (graph depicting average data of 4 replicates). (D) Measurements of total neurite length revealed significant elongation (5–6 replicates, n total=449–624) (E) as well as a shift of orientation towards the cathode (5–6 replicates, n total=449–624) when cells were stimulated at 25, 50, and 100V/m. The polar distribution of neurite angles (each dot represents the angle of a single neurite) demonstrates a significantly more prominent orientation towards the cathode at 50 and 100V/m. (F) Additionally, the 25 and 50 V/m DCEFs had a significant effect on the total length of the cell body (5–6 replicates, n total=493–688) when compared with cells exposed to 0 or 100V/m DCEF. (G) Polar distribution of cell body angles (each dot represents the angle of a single cell body) reveals that cell angles reorient parallel to the DCEF. (H-K) Photomicrographs of double phalloidin (F-actin, red) and DAPI (nucleus, blue) immunofluorescent staining illustrating the morphological and orientational changes in N2a cells in response to DCEFs (scale bar: 20 µm). (A-D, F) The values represent LS-means ± SEM. Statistical analyses were performed using ANOVA (split-plot-type) (A-D, F). (E) Kuiper’s Test of Uniformity and (G) chi-square test using grouped data (number of classes = 8 with identical divisions for interval 0–180°). *** P <.001 vs 0V/m; ## P <.01, ### P <.001 vs 25V/m; & P <.05, &&& P <.001 vs 50V/m. DCEF, direct current electrical field; GAP-43, growth associated protein-43; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LS, least square; OD, optical density; V/m, Volt/meter.
Mdck, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/custom%40ccl-34%4010%2E1128%2Fiai%2E01440-15?v=ATCC
Average 99 stars, based on 1 article reviews
mdck - by Bioz Stars, 2026-07
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murine microglial cell line nr-9460  (BEI Resources)
90
BEI Resources murine microglial cell line nr-9460
DCEF effects on neuronal elements. <t>N2a</t> mouse cells were differentiated into neuronal-like cells and various aspects relating to viability (A), morphology (B), and response (C) were assessed following exposure to increasing DCEFs (0, 25, 50, and 100V/m). (A) The total percentage of viable N2a cells (as measured in 3–4 replicates, n total=877–1900 cells) as well as the percentage of viable N2a cells specifically depicting neurites (5–6 replicates, n total=901–1134) was similar across all DCEFs applied (from 0 to 100V/m). (C) Protein concentration for GAP-43, a molecule expressed during axonal/neurite growth, was increased following a 24-hour stimulation of 25 and 50V/m (graph depicting average data of 4 replicates). (D) Measurements of total neurite length revealed significant elongation (5–6 replicates, n total=449–624) (E) as well as a shift of orientation towards the cathode (5–6 replicates, n total=449–624) when cells were stimulated at 25, 50, and 100V/m. The polar distribution of neurite angles (each dot represents the angle of a single neurite) demonstrates a significantly more prominent orientation towards the cathode at 50 and 100V/m. (F) Additionally, the 25 and 50 V/m DCEFs had a significant effect on the total length of the cell body (5–6 replicates, n total=493–688) when compared with cells exposed to 0 or 100V/m DCEF. (G) Polar distribution of cell body angles (each dot represents the angle of a single cell body) reveals that cell angles reorient parallel to the DCEF. (H-K) Photomicrographs of double phalloidin (F-actin, red) and DAPI (nucleus, blue) immunofluorescent staining illustrating the morphological and orientational changes in N2a cells in response to DCEFs (scale bar: 20 µm). (A-D, F) The values represent LS-means ± SEM. Statistical analyses were performed using ANOVA (split-plot-type) (A-D, F). (E) Kuiper’s Test of Uniformity and (G) chi-square test using grouped data (number of classes = 8 with identical divisions for interval 0–180°). *** P <.001 vs 0V/m; ## P <.01, ### P <.001 vs 25V/m; & P <.05, &&& P <.001 vs 50V/m. DCEF, direct current electrical field; GAP-43, growth associated protein-43; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LS, least square; OD, optical density; V/m, Volt/meter.
Murine Microglial Cell Line Nr 9460, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c8+d1a+murine+astrocytic+cell+lines/pmc10127605-259-1-6?v=BEI+Resources
Average 90 stars, based on 1 article reviews
murine microglial cell line nr-9460 - by Bioz Stars, 2026-07
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Image Search Results


DCEF effects on neuronal elements. N2a mouse cells were differentiated into neuronal-like cells and various aspects relating to viability (A), morphology (B), and response (C) were assessed following exposure to increasing DCEFs (0, 25, 50, and 100V/m). (A) The total percentage of viable N2a cells (as measured in 3–4 replicates, n total=877–1900 cells) as well as the percentage of viable N2a cells specifically depicting neurites (5–6 replicates, n total=901–1134) was similar across all DCEFs applied (from 0 to 100V/m). (C) Protein concentration for GAP-43, a molecule expressed during axonal/neurite growth, was increased following a 24-hour stimulation of 25 and 50V/m (graph depicting average data of 4 replicates). (D) Measurements of total neurite length revealed significant elongation (5–6 replicates, n total=449–624) (E) as well as a shift of orientation towards the cathode (5–6 replicates, n total=449–624) when cells were stimulated at 25, 50, and 100V/m. The polar distribution of neurite angles (each dot represents the angle of a single neurite) demonstrates a significantly more prominent orientation towards the cathode at 50 and 100V/m. (F) Additionally, the 25 and 50 V/m DCEFs had a significant effect on the total length of the cell body (5–6 replicates, n total=493–688) when compared with cells exposed to 0 or 100V/m DCEF. (G) Polar distribution of cell body angles (each dot represents the angle of a single cell body) reveals that cell angles reorient parallel to the DCEF. (H-K) Photomicrographs of double phalloidin (F-actin, red) and DAPI (nucleus, blue) immunofluorescent staining illustrating the morphological and orientational changes in N2a cells in response to DCEFs (scale bar: 20 µm). (A-D, F) The values represent LS-means ± SEM. Statistical analyses were performed using ANOVA (split-plot-type) (A-D, F). (E) Kuiper’s Test of Uniformity and (G) chi-square test using grouped data (number of classes = 8 with identical divisions for interval 0–180°). *** P <.001 vs 0V/m; ## P <.01, ### P <.001 vs 25V/m; & P <.05, &&& P <.001 vs 50V/m. DCEF, direct current electrical field; GAP-43, growth associated protein-43; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LS, least square; OD, optical density; V/m, Volt/meter.

Journal: International Journal of Neuropsychopharmacology

Article Title: The Morphological and Molecular Changes of Brain Cells Exposed to Direct Current Electric Field Stimulation

doi: 10.1093/ijnp/pyu090

Figure Lengend Snippet: DCEF effects on neuronal elements. N2a mouse cells were differentiated into neuronal-like cells and various aspects relating to viability (A), morphology (B), and response (C) were assessed following exposure to increasing DCEFs (0, 25, 50, and 100V/m). (A) The total percentage of viable N2a cells (as measured in 3–4 replicates, n total=877–1900 cells) as well as the percentage of viable N2a cells specifically depicting neurites (5–6 replicates, n total=901–1134) was similar across all DCEFs applied (from 0 to 100V/m). (C) Protein concentration for GAP-43, a molecule expressed during axonal/neurite growth, was increased following a 24-hour stimulation of 25 and 50V/m (graph depicting average data of 4 replicates). (D) Measurements of total neurite length revealed significant elongation (5–6 replicates, n total=449–624) (E) as well as a shift of orientation towards the cathode (5–6 replicates, n total=449–624) when cells were stimulated at 25, 50, and 100V/m. The polar distribution of neurite angles (each dot represents the angle of a single neurite) demonstrates a significantly more prominent orientation towards the cathode at 50 and 100V/m. (F) Additionally, the 25 and 50 V/m DCEFs had a significant effect on the total length of the cell body (5–6 replicates, n total=493–688) when compared with cells exposed to 0 or 100V/m DCEF. (G) Polar distribution of cell body angles (each dot represents the angle of a single cell body) reveals that cell angles reorient parallel to the DCEF. (H-K) Photomicrographs of double phalloidin (F-actin, red) and DAPI (nucleus, blue) immunofluorescent staining illustrating the morphological and orientational changes in N2a cells in response to DCEFs (scale bar: 20 µm). (A-D, F) The values represent LS-means ± SEM. Statistical analyses were performed using ANOVA (split-plot-type) (A-D, F). (E) Kuiper’s Test of Uniformity and (G) chi-square test using grouped data (number of classes = 8 with identical divisions for interval 0–180°). *** P <.001 vs 0V/m; ## P <.01, ### P <.001 vs 25V/m; & P <.05, &&& P <.001 vs 50V/m. DCEF, direct current electrical field; GAP-43, growth associated protein-43; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LS, least square; OD, optical density; V/m, Volt/meter.

Article Snippet: Murine N2a neuroblastoma (ATCC, Burlington, ON, Canada), BV2 microglial (gift from Dr. Serge Rivest, CRCHUQ), and C8-D1A astrocytic cells (ATCC) were seeded at densities of 1.36×10 4 cells/cm 2 for N2a and BV2 cells and 3.64×10 4 cells/cm 2 for C8-D1A cells, taking into account cell division rates of each cell type.

Techniques: Protein Concentration, Staining

Summary of Statistical Correlations

Journal: International Journal of Neuropsychopharmacology

Article Title: The Morphological and Molecular Changes of Brain Cells Exposed to Direct Current Electric Field Stimulation

doi: 10.1093/ijnp/pyu090

Figure Lengend Snippet: Summary of Statistical Correlations

Article Snippet: Murine N2a neuroblastoma (ATCC, Burlington, ON, Canada), BV2 microglial (gift from Dr. Serge Rivest, CRCHUQ), and C8-D1A astrocytic cells (ATCC) were seeded at densities of 1.36×10 4 cells/cm 2 for N2a and BV2 cells and 3.64×10 4 cells/cm 2 for C8-D1A cells, taking into account cell division rates of each cell type.

Techniques:

Responses of Cells of the CNS to DCEF

Journal: International Journal of Neuropsychopharmacology

Article Title: The Morphological and Molecular Changes of Brain Cells Exposed to Direct Current Electric Field Stimulation

doi: 10.1093/ijnp/pyu090

Figure Lengend Snippet: Responses of Cells of the CNS to DCEF

Article Snippet: Murine N2a neuroblastoma (ATCC, Burlington, ON, Canada), BV2 microglial (gift from Dr. Serge Rivest, CRCHUQ), and C8-D1A astrocytic cells (ATCC) were seeded at densities of 1.36×10 4 cells/cm 2 for N2a and BV2 cells and 3.64×10 4 cells/cm 2 for C8-D1A cells, taking into account cell division rates of each cell type.

Techniques: Derivative Assay, Modification, Migration, Expressing

Summary of Results

Journal: International Journal of Neuropsychopharmacology

Article Title: The Morphological and Molecular Changes of Brain Cells Exposed to Direct Current Electric Field Stimulation

doi: 10.1093/ijnp/pyu090

Figure Lengend Snippet: Summary of Results

Article Snippet: Murine N2a neuroblastoma (ATCC, Burlington, ON, Canada), BV2 microglial (gift from Dr. Serge Rivest, CRCHUQ), and C8-D1A astrocytic cells (ATCC) were seeded at densities of 1.36×10 4 cells/cm 2 for N2a and BV2 cells and 3.64×10 4 cells/cm 2 for C8-D1A cells, taking into account cell division rates of each cell type.

Techniques: